The MOUSE ANTI HUMAN p53 (aa181-190) Antibody from MyBioSource.com is a Mouse Monoclonal antibody to Antigen NY-CO-13, bbl, LFS1, Mutant p53, TP53, and tumor protein p53. This antibody recognizes Human antigen. The MOUSE ANTI HUMAN p53 (aa181-190) Antibody has been validated for the following applications: Immunohistochemistry, Immunoprecipitation, and Western Blot.
Description
Mouse anti Human p53 antibody, clone DO-11 recognizes the human Cellular tumor antigen p53, also known as p53 tumour suppressor protein or NY-CO-13. p53 is a 393 amino acid ~53kDa cytoplasmic/ nuclear protein up-regulated in response to DNA damage and is found in a wide variety of transformed cells (UniProt: P04637). DO-11 binds to an epitope within the central region of p53 between amino acids 181 - 190 defining a cryptic epitope exposed in unfolded/ denatured p53 (Shimitzu et al. 2006). Nine isoforms of human p53 are produced by alternative splicing and promotor usage, the epitope recognized by clone DO-11 is present in all isoforms. Mouse anti Human p53 antibody, clone DO-11 recognizes mutant forms of p53 (Warnock et al. 2011) and has been used successfully for detection of p53 by western blotting (Wei et al. 2012)